ABSTRACT
OBJECTIVE@#To investigate the clinical efficacy of a modified paramedian lower lip-submandibular approach for maxillary (subtotal) total resection.@*METHODS@#Eleven patients of maxillary tumors underwent maxillary (subtotal) total resection through the modified paramedian lower lip-submandibular approach. Clinical follow-up visits were conducted to evaluate appearance restoration, facial nerve functional status, parotid gland functional status, and orbital region complication.@*RESULTS@#During the follow-up period of 6-36 months, the appearance of all 11 patients recovered well. All cases presented hidden scars. No facial nerve and parotid duct injury, lower eyelid edema, lower eyelid ectropion, or epiphora in all cases was observed.@*CONCLUSIONS@#Applying modified paramedian lower lip-submandibular approach to maxillary (subtotal) total resection effectively reduces incidence of orbital region complications including lower eyelid edema, lower eyelid ectropion, and epiphora, which often occur to traditional approach. The modified approach produces more subtle scars than other methods and should be applied to treatment of maxillary (subtotal) total resection.
Subject(s)
Humans , Facial Nerve , Lip , Maxilla , Maxillary Neoplasms , Surgical FlapsABSTRACT
BACKGROUND:Bilateral ovariectomy is a most commonly used method to establish an animal model of postmenopausal osteoporosis.Up to now,little is reported on the C57 mouse model of osteoporosis with calvarial critical-size defects.OBJECTIVE:To establish the C57 mouse model of osteoporosis with calvarial critical-size defects.METHODS:Thirty-six C57 mice aged 8 weeks were randomly divided into two groups:the mice in model group received bilateral ovariectomy,and the same weight of fat tissues around the ovaries of the other mice were cut as control group.At 6 weeks after modeling,the mouse femur was removed for micro-CT scan and hematoxylin-eosin staining to testify whether there is a success or failure in animal modeling.Afterwards,six mice were respectively selected from each group,and two round calvarial defects with a diameter of 4 mm were symmetrically made on the parietal bone.The defect healing was evaluated by micro-CT scan at 8 weeks after modeling.RESULTS AND CONCLUSION:At 6 weeks after modeling,the bone volume fraction,bone surface to volume ratio,trabecular thickness,trabecular number,trabecular spacing and bone mineral density in the modeling group were significantly lower than those in the control group (P ＜ 0.05).In the model group,there were loosen or fractured trabeculae,and enlarged medullary cavity.At 8 weeks after bone defects,showed no significant micro-CT changes in the defect region in both two groups.These findings implicate a success in establishing the C57 mouse model of osteoporosis with calvarial critical-size defects.
ABSTRACT
BACKGROUND:To obtain enough adipose-derived stem cells (ADSCs) is the premise of repairing osteoporotic bone defects by autograft transplantation;therefore,how to efficiently,simply and economically harvest primary autologous ADSCs is the key to the treatment of osteoporosis.OBJECTIVE:To isolate and culture ADSCs from mice with osteoporosis (OP-ADSCs) in vitro by tissue explant culture and collagenase digestion,and to compare the efficacies of these two methods.METHODS:Adipose tissues were isolated from the inguen of C57BL/6 mice,from which OP-ADSCs were obtained by tissue explant culture and collagenase digestion respectively.Then,the cells were subjected to primary culture and subculture.The surface specific antigens of passage 3 cells were observed using flow cytometry,while the yields and proliferation abilities of passage 3 were compared at 7 days of culture.The adipogenic and osteogenic differentiation of OP-ADSCs at passage 4 was detected.RESULTS AND CONCLUSION:(1) The expression levels of CD34,CD146,Sca-1 in passage 3 cells were (15.22±1.85)％,(75.55±3.36)％ and (83.48±4.22)％ for the tissue explant culture and (13.46±2.21)％,(76.62±2.47)％ and (84.84±3.56)％ for the collagenase digestion method,respectively.(2) The cell yield from each milligram of adipose tissue by tissue explant culture was significantly higher than that by collagenase digestion (P ＜ 0.05).(3) The proliferation rate of cells in the tissue explant culture group was higher than that in the collagenase digestion group after 24 hours of culture.(4) The OP-ADSCs cultured by the two methods could differentiate into adipocytes and osteoblasts,and the lipid accumulation and the mineralized nodules showed no significant difference between the two groups (both P＞ 0.05).These experimental findings indicate that the tissue explant culture is more suitable for obtaining OP-ADSCs in vitro as compared with collagenase digestion,which contributes to provide adequate cell sources for studies on ADSCs treatment of osteoporotic fractures and bone defects.